you can access the Chemical Shift Mapping tutorial either in the Help Menu in the programme: Help / Tutorials / Chemical Shift Perturbation Tutorial or from the website: https://www.ccpn.ac.uk/v3-software/tutorials/chemical-shift-perturbation-analysis-tutorial/view This will take you through how to set up a titration series and analyse the data. The macro above is just an alternative way to transfer the assignments from one peak to another, if (like me) you are not so keen to do it with the system outlined in the tutorial. It is worth pointing out, though, that V2 does have a "Follow Shift Changes" routine which we don`t yet have in V3. So transferring the assignments from one spectrum to another is slightly less automated in V3 compared to V2 at the moment.
Best wishes,
Vicky
Hi Vicky (I am actually the person who asked this question , above, but was unable to re-access the account so started under a new user name :)). I see the tutorial. Apologies, I don`t understand why I want to drop my original Apo peak list onto the final "eqb" spectrum when I want to follow the shift change and read out the peak list of the shift changes correlated with each other? Wouldn`t that delete my peak list for my original apo data? Just wondering "Will Follow Shift Change" be implemented at all? I don`t have a BMRB assignment for this particular data (hoping to collect a C13N15). This is an HSQC experiment only looking at titrations. I want to calculate Kds from the peak shifts etc at different concentrations fro different peaks.