Hi Tess,
glad things are working out better on NMRbox.
It looks like there is still a problem with at least one Glycine:
I wonder if this is stopping the correct chemical shift file from being written for TALOS and then the rest of the errors just start piling up.
In terms of your Glycine HAs: if you can see peaks at two distinct chemical shifts, then you should name these HAx and HAy (i.e. the chemical shifts are not degenerate, but you can’t make a stereospecific assignment). If you only see one chemical shift, then you should use HA% (i.e. the two HA chemical shifts are degenerate and so any peaks contain information about both HAs). Note that HA% in NEF format will get changed to HA# in XPLOR/TALOS format. Might you accidentally have both an HAx and an HA% for Gly 118? Sometimes you do get situations where the HAx and HAy chemical shifts are quite close and so you can distinguish them in some spectra, but in others (with lower resolution) you can’t. In that situation you should make sure that the combined peak is labelled as being assigned to both HAx and HAy, rather than HA%. You shouldn’t ever have a situation where you have HAx, HAy and HA% for a single residue - it should always be either HAx and HAy OR HA%.
Hope that will help you sort things out.
Vicky