Hello,
I have a doubt on the different y-axis plot options after the CSP calculations. Could you please provide a reference or an explanation to the y axis plot options?
I am not sure what to plot to see the delta delta comb (as by your titration manual - https://www.ccpn.ac.uk/manual/v3/Titrations.html. Is the “delta delta” the correct option?
Many thanks,
Francesca
Hi Francesca,
yes, Delta delta is usually what people want to plot - this is the change (usually denoted by a capital Delta) in the chemical shift (usually denoted by a lower case Delta).
In theory you can choose lots of other columns from the table. Many of them don’t make much sense, of course. But something like the Kd could be interesting to see how the fit compares across different residues.
Vicky
Hi Vicky,
that’s perfect! This is actually what I usually plot - but I was puzzled by all the options and wanted to make sure I did understand correctly.
Many thanks,
Francesca