Exporting/Importing .nef files for CYANA calculations

Analysis Version 3
4

Hello Anne and Eliza,

Thanks for the above information - I had similar problems importing NEF file into CYANA.

I have my 1D proton, TOCSY and ROESY spectra imported into CCPN to pick the peaks in mainly TOCSY and ROESY spectra. I cleaned up the chemical shift list before exporting to remove the same shift picked in different spectra, and I left some of the aromatic peaks unassigned. I only exported the chain, the chemical shift list and the ROESY peak list. When I was importing the NEF file, CYANA could not read the chemical shift list (see the screenshot).

Screen Shot 2023-08-06 at 2.38.35 pm
Screen Shot 2023-08-06 at 2.38.35 pm1224×708 31.1 KB

Thanks for any help in advance!