Hi Ken,
it looks like the only equation we currently have implemented is not the one from Williamson (2013), but based on a more simple one-site binding model:
y = Bmax*x/(Kd + x)
where Bmax is the maximum binding. Hence the protein concentration isn`t used.
See https://www.graphpad.com/guides/prism/8/...ecific.htm for an example.
We will be implementing other equations in future (indeed at some point even a system so you can specify your own equation). In the mean time if you want to do a different kind of fitting you will have to export the data to another programme.
See https://www.ccpn.ac.uk/manual/v3/WorkingWithTables.html for details on exporting tables and also editing the columns shown etc.
Best wishes,
Vicky