How is the possibility for the best match of the ‘i+1’ or ‘i-1’ calculated?

Analysis Version 3
#1

Hi there,
I am using your 3.0.4 version in order to be able to use the IBS Macros. I am doing backbone assignments and I am wondering how the possibility for the best match of the ‘i+1’ or ‘i-1’ is calculated, or on what the calculation is based on. I have this feeling sometimes, that the matching of my CO peaks are not influencing the prediction. Could you please explain how the calculation works? I remember that in the version 2.5 the possibility was calculated in terms of how many peaks are matching, there was a number of matching peaks given.

Thank you for the software support and the help

Lukas

#2

Hi Lukas. The CO peaks are not used by default you have to go into the options for the backbone assigner and check the appropriate option. There’s a little bug as well in that you have to uncheck and re-check this option each time you start the backbone assigner.

#3

Hi bosmith,
thank you for your answer! I could finally get rid of this bug, but it appears to me , that the CO is not impacting the best match score much (if I uncheck it it has nearly no influence on the score), which is kind of sad for my assignment. Also I only see 6 possible stripes of the score. Is there a possibility to see the score for all residues in a table like in CCPN 2.5?

Thanks again for your help!

Lukas