Is there any feature which can help me with the indirect referencing for my 15N data? I tried to use the calibration from peaks but I am not confident to choose which peak that might not be massively perturbed. I tried with different location of the peak and it gave me slightly different CSP data.
Regards
Are you then looking for a global alignment routine?
Yes, sort of.
Hi Ireno,
If you are trying to look at CSPs, then really all the spectra should have been recorded with the same frequencies/offsets. The spectra should then all be referenced in the same way and not be moved relative to one another. If your 15N referencing is way off for some reason or other you can change this in the Spectrum Properties Dimensions tab (change the Referencing (ppm) value). But make sure this value is the same for all spectra.
The calibration from peaks is more for the situation where you are using spectra that were recorded on different spectrometers, for example, and you need to make sure that there is some internal consistency between the spectra. But to my mind, for looking at CSPs you precisely don’t want this.
Hope that helps,
Vicky
Dear Vicky,
My problem is that the referencing (ppm) value were completely different for two spectrum. My first spectrum ( wild type) shows 1H 8.789 and 15N 116.625. Second spectrum shows 1H 14.721 and 15N 134.146. When I changed the value of 2nd to the 1st, the overlaid far away.
