Strange Appearance of 3D Specta

Analysis Version 3
1

I have imported the standard set of 3D experiments for assignments, as well as a corresponding 2D- 15N-HSQC.

Originally, all the spectra looked fine, but now when opening them, they look very weird. For instance, they are just noise with straight lines cutting across the spectrum.

The spectra are still mapped correctly, and if I import the same spectra file (xyz.pipe) as a new spectrum, it works.

2

My first thought is that this could be an issue with contour levels. Have a look at the actual contour level values with CO. Try changing the contour levels of the weird spectrum in order of magnitude steps and see if things improve.

Vicky

3

To my mind straight lines going across the spectrum sounds like a mismatch in datapoint sizes or some like, can you send a picture?

Regards
Gary

Dr Gary S Thompson NMR Facility Manager
CCPN CoI & Working Group Member
Wellcome Trust Biomolecular NMR Facility
School of Natural Sciences
University of Kent, Canterbury, Kent, England, CT2 7NZ

:telephone::01227 82 7117
:envelope:: g.s.thompson@kent.ac.uk
orchid: ORCID

4

Thank you very much for the quick response. below are representatives of how the spectra look.

image
image847×724 218 KB

image
image888×742 106 KB

And increasing the contours by 8 orders of magnitude fixed it.

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